Artificial Breeding Technology of Allogynogenetic crucian carp

First, broodstock
1. The broodstock selection female parent is Heilongjiang Fangzheng or its hybrid offspring (Alternative Silver Juvenile), and the father is the Xingguo Red Dragonfly of Jiangxi Province. The inclusion criteria were as follows: 30-32 female parent line scales, small head, high body height, thick back, strong physique, no injury, sickness, and body weight above 250 grams; paternity requirement for body height, back thickness, strong appearance, body color red Yan, weighing 1 kg to 1.5 kg.
2. The area of ​​the pro-fish pond is 1 a mu to 2 mu, and the water depth is 1.5 to 2 meters. The bottom of the pond is flat and it is convenient for drainage and irrigation. The pro-fish ponds must be completely cleared of ponds before stocking to prevent all kinds of wild fish from mixing in and removing the weeds from the pond to prevent the brooders from spawning themselves.
3. The quality and quality of broodstock production is related to the development of broodstock breeding. When the broodstock is cultivated vigorously, the amount of eggs is large, the egg quality is good, and production is easy to produce. The fertilization rate and hatching rate are relatively high. After the broodstock was collected in autumn and winter, the gonads were graded according to their gonad development status and gender-specific cultivation. Until the beginning of March, they will be sorted once again to eliminate the wild fish and to prevent the miscellaneous breeding of silver and silver. The mother stocking density is 1500 kg/m2 to 2250 kg/m2.
(1) The cultivation of broodstock in autumn and winter should be intensively cultivated from autumn to promote the development of gonads. Feeds such as bean cakes and wheat are preferred. The size of the compound feed is palatable. Daily feeding is about 5% of the total weight of the fish, and the amount of feed should be reduced after winter.
(2) After the spring in spring, the amount of fish fed is gradually increased by 3% to 5% of the total weight of the fish. Malts, snails, etc. can be added, and new water can be added appropriately to promote appetite and gonadal development and maturation of the broodstock. However, when the breeding season is approaching, new water must not be added, otherwise it is easy to induce miscarriage.
(3) postpartum broodstock bred postpartum broodstock physique weakness, need intensive care. It requires fresh water, adequate food, and a quiet environment.
Second, reproduction
1. The breeding season mastering breeding season is similar to that of pupa and pupa. The area around the Yangtze River is generally from early April to late May. The water temperature can be used for oxytocin production at 18°C ​​to 28°C. When the temperature of the water just rises to 18°C ​​to 20°C, it is not necessary to rush to produce. It takes more time after one week's cultivation to carry out artificial oxytocin production. During the breeding season, we must pay close attention to the weather forecast, prevent the drastic changes in temperature, observe the fishery in the season of production, and prevent a large number of abortions.
2. Selection of spawning broodstock Selection of broodstock with good sexual maturity is the key to increasing productivity, spawning, and quality fry. The female's mature brood's abdomen expands and is softly and elastically pressed. The abdomen of the flipped fish body is clearly recessed and the vents are ruddy. The eggs can be seen with slight pressure. For example, if eggs are inspected with an egg dicer, the eggs are yellow-green, uniform in size, and separated from each other, indicating that they have matured and should be produced promptly. If the eggs are not uniform in size, they are grayish and gray with white ovules, indicating that the eggs have not yet been found. Mature, should not elicit production.
3. Induction (1) Use of oxytocicants Individuals weighing 250 grams or more were divided into 2 injections. The first injection of LRH-A 1.5 micrograms to 2 micrograms was performed at the first injection. The second needle was injected every 5 hours to 6 hours. Each tail was injected with LRH-A 1.5 micrograms to 2.5 micrograms and PG2 micrograms to 3 micrograms. The first dose at fractional injection was 1/4 to 1/3 of the total dose. Whether or not the broodstock is injected with oxytocin depends on the development condition. Generally, no mature injection is required. If the semen is insufficient, half of the female dose of oxytocic acid can be injected per kilogram of body weight. One injection of the female second injection is completed. The female fish was injected at about 5 pm and injected into the body at the base of the pectoral fin.
(2) Mastering the broodstock after injection and controlling the time of effect The injected female and male broodstock can be kept separately in cages, if micro-flowing water is better. When the water temperature is 18°C ​​to 28°C, the effect time of oxytocin production is generally 12 hours to 17 hours. Near the effect time, the broodstock jumped in the cage and was extremely excited. At this time, the broodstock should be checked. If the broodstock is brought up, the eggs will flow out automatically or slightly out of pressure, and artificial insemination should be performed immediately.
4. Insemination (1) Wet insemination Take a clean small bowl or bowl, and pour approximately 100 ml of 0.75% physiological saline first. Then squeeze a small amount of semen to mix well. Squeeze the egg in the bowl and squeeze the egg. Stir and replenish the semen. Detackify after 3 minutes. When debonding, slowly transfer the fertilized egg into pre-prepared muddy water or talcum powder, and agitate in time so that the egg does not sink or clump. After 5 minutes, scouring, rinsing, and then hatching the eggs into a hatchery or hatching ring. The fertilized egg can also not be detacked, so that it adheres to the fish nest (the fish nest is bundled and can resist decay), and the fish nest is incubated in a pool or water flowing through the clear pond.
(2) Dry insemination Dry insemination should maintain the “three dryness”, that is, the container should be dry, the fish should be dry and the hand should be dry. Otherwise, the sperm can quickly lose its vitality when it encounters water, and the egg can also produce stickiness when it meets water, and it cannot achieve good results. Insemination effect. After preparing for the "Three Dry," quickly squeeze eggs and squeeze semen into the container, and gently stir it. After 3 minutes, detackify as above.
(3) Natural spawning The broodstock after the fertilization of the hormone is injected into the spawning pool at a ratio of 1:1 or 2:1. A fish nest is set around the pool and the fertilized eggs adhere to it. Then the nest with the eggs is hung in the pool that passes through the clear pond. The nest can be located about 25 cm below the surface of the water.
(4) Preparation of yellow mud water and talcum powder
One kilogram of fine yellow mud with 5 litres of clean water is used to filter the rejects after pulping. Take 100 grams of medical talcum powder plus salt 10 grams (without plus), with 5 liters of water to stir.
Third, hatching deferred fertilized eggs should be sent to hatching barrel or hatching ring as soon as possible. The density of flowing eggs for hatching was 800,000 grains/m3 to 1 million grains/m3, and the flow rate should not be too large. The eggs could be turned upside down and dispersed. Non-debonded nests can be hung in the hatchery or in the hatchery ring, or they can be hung in the pool in the clear pond. The length of fry hatching is mainly determined by the temperature of the water. When the water temperature is 18°C, the membrane hatches after about 100 hours to 120 hours, and about 60 hours to 65 hours at 22°C. When the fry water temperature is between 20°C and 22°C after filming, the fry chamber is inflated 4 days later. At this time, the pond can be cultivated or sold.

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