Reasonable use of fish oxytocin
2018-05-23 16:00:42
Reasonable use of oxytocin is one of the keys to the success of artificial breeding of fish. There are currently three kinds of oxytocin commonly used in artificial reproduction of fish: human chorionic gonadotropin (HCG), luteinizing hormone-releasing hormone analogue (LRH-A), and pituitary PG of fish. This article describes the use of the first two oxytocin agents.
First, the use of two fish oxytocants
1. Human chorionic gonadotropin (HCG) is one of the major oxytocin drugs in the production of artificial breeding fry and cod. Its finished product is white or light yellow powder, soluble in water, and it is colorless or light yellow liquid in water. Its aqueous solution is difficult to preserve, and its storage time is generally less than 24 hours, and it is easy to fail when exposed to heat. Therefore, the product should be sealed and stored in a cool, dry place or in a refrigerator.
Usage: (1) Dosage: Calculated according to the broodstock per kilogram body weight, the female fish injection volume is 800-1200 international units, and the male fish is halved. (2) Preparation of Hormone Injection: Firstly, prepare physiological saline (8 g of sodium chloride plus distilled water or 1000 ml of cold boiled water to fully dissolve). Sterilize the syringe and needle before use. When using, draw a fixed amount of physiological saline with a syringe, insert the needle into the rubber stopper of the hormone bottle, and inject the physiological saline, shake it for 2-3 minutes, and after the hormone is fully dissolved, suck it out for use. (3) Injection: One person tilts the fish sideways to expose the injection site to the water, and the other person holds the needle. Before the injection, the water at the injection site should be wiped off, and after disinfected with 70% alcohol or iodine, the needle should be pierced into the chest cavity at an angle of 45 degrees above the fish body surface and slowly injected into the liquid. One injection method is generally used.
2. Luteinizing Hormone Releasing Hormone Analogues (LRH-A): The biological activity of LRH-A is tens of times higher than that of LRH (Luteinizing Hormone Releasing Hormone), and it is fully matured for gonadal development. Fish, oysters and other fish have oxytocin, which can lead to the maturation of female eggs, ovulation and male sperm production. The order of susceptibility of LRH-A to various oyster-producing fish is: grass carp> grasshopper> earthworm> earthworm. Dosage: When the water temperature is 19°C-29°C, the LRH-A can stimulate the production of grass carp. The effective dose for one injection is 10-30 μg/kg body weight of fish. When the water temperature is 24°C-27°C, the spasm can be induced by one injection. The effective dose is 50-60 micrograms per kilogram of fish body weight; when the water temperature is 25 degrees Celsius-31 degrees Celsius, the effective production dose is 30-60 micrograms per kilogram of fish body weight.
The time from the time of LDH-A oxytocin injection to fish, that is, the time from the injection of oxytocic acid to the estrus of the broodstock, was delayed by 3-4 hours or even longer than that of the injection of human chorionic gonadotropin and pituitary gland. The reason is that LRH-A and HCG, PG have different ways of oxytocin.
3. Efficient oxytocin mixture is currently produced on the market with an efficient oxytocin mixture, which is produced from commonly used oxytocins and the addition of catecholamines, RES and other chemical substances. According to the determination, RES and other substances can increase the content of GTH in the broodstock serum, promote the development and ovulation of egg cells, shorten the effect time of the drug, and stabilize the effect of oxytocin production.
Second, the choice of fish oxytocin The sensitivity of different types of broodstock to different oxytocin has great differences, in the oxytocin must be selected for the broiler most sensitive oxytogen, or a combination of several oxytocin to achieve Satisfy oxytocin effect. Based on the relevant information and our personal experience, we will introduce you here. (1) Sputum and sputum: The sensitivity to HCG is better than that of LRH-A; the amount of HCG is 1000-1200 IU/kg. (2) Grass carp: The sensitivity to HCG is poor, LRH-A is preferred, dosage is 15-20 μg/kg, PG, 5 mg/kg, or a combination of both. (3) Herring: It is difficult to work with HCG alone. Must be a mixed injection of HCG and PG or PG alone. (4) Head lice: The sensitivity to HCG is better than that of LRH-A, the amount of HCG is 1000-15000 IU/kg, and PG 7-10 mg/kg is also available. (5) Cockroaches: Use of combination drugs is more often used for each kg of fish body weight: PG (2-4 mg) + HCG (100-300 IU); LRH-A (10-20 μg) + HCG (500-600 IU). Some fish, such as silver cockroaches and yellow tails, all use PG alone.
Third, the injection time of oxytocin The injection time of fish oxytocic acid should be determined according to the time of effective delivery and the planned spawning time. If it is required to collect fish eggs at 6 o'clock in the morning and the oxytocin has a time of 12 hours, the injection time should be 6 o'clock in the evening of the previous day. When HCG and PG oxytocin are used, the time of effect of the home fish is: 16-18 hours at 19°C-20°C; 12-14 hours at 22°C-23°C; 9-10 hours at 26°C-27°C . However, the use of LRH-A will take three to three hours to delay.
First, the use of two fish oxytocants
1. Human chorionic gonadotropin (HCG) is one of the major oxytocin drugs in the production of artificial breeding fry and cod. Its finished product is white or light yellow powder, soluble in water, and it is colorless or light yellow liquid in water. Its aqueous solution is difficult to preserve, and its storage time is generally less than 24 hours, and it is easy to fail when exposed to heat. Therefore, the product should be sealed and stored in a cool, dry place or in a refrigerator.
Usage: (1) Dosage: Calculated according to the broodstock per kilogram body weight, the female fish injection volume is 800-1200 international units, and the male fish is halved. (2) Preparation of Hormone Injection: Firstly, prepare physiological saline (8 g of sodium chloride plus distilled water or 1000 ml of cold boiled water to fully dissolve). Sterilize the syringe and needle before use. When using, draw a fixed amount of physiological saline with a syringe, insert the needle into the rubber stopper of the hormone bottle, and inject the physiological saline, shake it for 2-3 minutes, and after the hormone is fully dissolved, suck it out for use. (3) Injection: One person tilts the fish sideways to expose the injection site to the water, and the other person holds the needle. Before the injection, the water at the injection site should be wiped off, and after disinfected with 70% alcohol or iodine, the needle should be pierced into the chest cavity at an angle of 45 degrees above the fish body surface and slowly injected into the liquid. One injection method is generally used.
2. Luteinizing Hormone Releasing Hormone Analogues (LRH-A): The biological activity of LRH-A is tens of times higher than that of LRH (Luteinizing Hormone Releasing Hormone), and it is fully matured for gonadal development. Fish, oysters and other fish have oxytocin, which can lead to the maturation of female eggs, ovulation and male sperm production. The order of susceptibility of LRH-A to various oyster-producing fish is: grass carp> grasshopper> earthworm> earthworm. Dosage: When the water temperature is 19°C-29°C, the LRH-A can stimulate the production of grass carp. The effective dose for one injection is 10-30 μg/kg body weight of fish. When the water temperature is 24°C-27°C, the spasm can be induced by one injection. The effective dose is 50-60 micrograms per kilogram of fish body weight; when the water temperature is 25 degrees Celsius-31 degrees Celsius, the effective production dose is 30-60 micrograms per kilogram of fish body weight.
The time from the time of LDH-A oxytocin injection to fish, that is, the time from the injection of oxytocic acid to the estrus of the broodstock, was delayed by 3-4 hours or even longer than that of the injection of human chorionic gonadotropin and pituitary gland. The reason is that LRH-A and HCG, PG have different ways of oxytocin.
3. Efficient oxytocin mixture is currently produced on the market with an efficient oxytocin mixture, which is produced from commonly used oxytocins and the addition of catecholamines, RES and other chemical substances. According to the determination, RES and other substances can increase the content of GTH in the broodstock serum, promote the development and ovulation of egg cells, shorten the effect time of the drug, and stabilize the effect of oxytocin production.
Second, the choice of fish oxytocin The sensitivity of different types of broodstock to different oxytocin has great differences, in the oxytocin must be selected for the broiler most sensitive oxytogen, or a combination of several oxytocin to achieve Satisfy oxytocin effect. Based on the relevant information and our personal experience, we will introduce you here. (1) Sputum and sputum: The sensitivity to HCG is better than that of LRH-A; the amount of HCG is 1000-1200 IU/kg. (2) Grass carp: The sensitivity to HCG is poor, LRH-A is preferred, dosage is 15-20 μg/kg, PG, 5 mg/kg, or a combination of both. (3) Herring: It is difficult to work with HCG alone. Must be a mixed injection of HCG and PG or PG alone. (4) Head lice: The sensitivity to HCG is better than that of LRH-A, the amount of HCG is 1000-15000 IU/kg, and PG 7-10 mg/kg is also available. (5) Cockroaches: Use of combination drugs is more often used for each kg of fish body weight: PG (2-4 mg) + HCG (100-300 IU); LRH-A (10-20 μg) + HCG (500-600 IU). Some fish, such as silver cockroaches and yellow tails, all use PG alone.
Third, the injection time of oxytocin The injection time of fish oxytocic acid should be determined according to the time of effective delivery and the planned spawning time. If it is required to collect fish eggs at 6 o'clock in the morning and the oxytocin has a time of 12 hours, the injection time should be 6 o'clock in the evening of the previous day. When HCG and PG oxytocin are used, the time of effect of the home fish is: 16-18 hours at 19°C-20°C; 12-14 hours at 22°C-23°C; 9-10 hours at 26°C-27°C . However, the use of LRH-A will take three to three hours to delay.