Part of the "safety" points of the biosafety cabinet purchase

The biosafety cabinet is a vacuum filtration fume hood for biosafety protection in biosafety laboratories and other laboratories, which prevents operators and the environment from being exposed to bioaerosols generated during the experiment; for operators, samples And cross-contamination between samples and the environment provides safety protection. At present, there are many brands of biosafety cabinets on the market, the quality of the products is not uniform, and it is also very irregular, which causes many inconveniences for the purchase of users. As a well-known biosafety cabinet brand in the Asian region, Heal Force brand has become one of the internationally renowned biosafety cabinet brands. It is the main product of the YY0569-2005 biosafety cabinet in the pharmaceutical industry of the People's Republic of China. Participate in the unit. We will continue to introduce some articles about the biosafety cabinet to share with you, and hope to help the teachers to pay attention to when purchasing biosafety cabinets.

The purpose of purchasing a biosafety cabinet is to ensure a safe experimental operating environment, so the only requirement for a safety cabinet is “safety”. With the mandatory implementation of the mandatory standard “YY0569-2005” for the biosafety cabinet of the pharmaceutical industry of the People's Republic of China on June 1, 2006, it will play an important role in regulating the market and ensuring the safety performance of the biosafety cabinet. Below we will introduce "What kind of safety cabinet is truly safe" around the key points of the new national industry standards and the choice of safety cabinets.

First, choose the appropriate safety cabinet level, type

Determine the appropriate type of cabinet based on the hazards of the experiment you are undertaking. At present, most laboratories (including P3 laboratories) have purchased secondary biosafety cabinets that are fully capable of meeting daily work needs and safety protection needs; depending on whether you will use volatile toxic chemicals in your experiments or The radionuclide and the amount of the radionuclides are used to determine the choice of a Class A2 or Class B2 cabinet.
National industry standards stipulate that A2 safety cabinets can be used for microbial experiments with trace volatile toxic chemicals and trace radionuclides as adjuvants, but efflux pipes must be installed. If the amount of toxic chemicals or radionuclides is large, a fully vented B2 safety cabinet should be used.

Second, choose a truly "safe" biological safety cabinet

Biosafety cabinets are fundamentally based on the rational use of high-efficiency filtration membranes and airflow patterns to protect operators, protect samples, and protect the environment. The airflow mode is ensured by the structure of the safety cabinet and important components such as fans. Therefore, we believe that the core points of the truly “safe” biosafety cabinet are: high efficiency filtration membrane and safety cabinet. The cabinet structure and air tightness, the third is the anti-leak test of the biosafety cabinet. The third is the optimized airflow rate and excellent fan. The fourth is an essential alarm function. Let's discuss these security elements one by one.
1. High-efficiency air filter filtration effect The high-efficiency/ultra-efficient air particle filter (HEPA/ULPA) installed in the biosafety cabinet is composed of a disposable filter membrane with extended/pleated media supported by a hard outer casing. The main purpose is three:
(1) Filtering dust and other particles in the air flowing into the working area of ​​the safety cabinet to ensure that the air in the safety cabinet reaches the specified cleanliness.
(2) Filter out dangerous particles such as aerosols generated during the test to protect the sample and avoid cross-contamination between samples.
(3) Prevent dangerous particles in the air discharged from the safety cabinet from being directly discharged into the environment. To this end, the industry has established industry standards for the filtration effectiveness and integrity of safety cabinets. For particles with a diameter of 0.3 μm, the filtration efficiency of the high-efficiency filter is not less than 99.99%; in terms of detecting the integrity of the filter sealing effect, it is stipulated that the leakage rate of the filter that can be scanned and detected should not exceed at any point. 0.01%, the filter that cannot be scanned for detection should not exceed 0.005% at the detection point. Only when these requirements are met can the filter be safely and effectively filtered by the dust in the air flowing into and out of the safety cabinet and the aerosol generated during the test, thereby realizing the safety effect of the safety cabinet. The high-efficiency air filter filter membrane installed in the Heal Force HF safe biosafety cabinet is made of borosilicate fiberglass. The support shell and the separator are made of aluminum alloy. 100% relative humidity and temperature, corrosiveness and mechanical strength under test conditions, the filtration efficiency of particles with a diameter of 0.3 microns in air is 99.999%. Each biosafety cabinet needs to be scanned and tested by the filter before leaving the factory. Leakage rate test, the performance exceeds the national industry standards, fully guarantees the filtering effect of the flowing air in the safety cabinet, and ensures the safety protection of the protected object. At the same time, the Heal Force safety cabinet is equipped with the world* filter life display bar code, which can display the life of the high-efficiency filter accurately and in real time, and provide an alarm before the filter fails to remind the user to prepare for replacement in advance. It will not delay the progress of the test due to filter damage, and it will provide better protection for the safety of the safety cabinet.
2. The cabinet structure of the safety cabinet and the cabinet structure and airtightness of the airtight safety cabinet are also important aspects related to the safety of the safety cabinet. China YY0569-2005 safety cabinet standard stipulates that the working area of ​​the second-class A2, B1, B2 safety cabinets should adopt four-layer structure (left and right sides, rear part, bottom part), and the pollution parts of all levels of safety cabinets should be negative. The pressure state is surrounded by a negative pressure passage and a ventilation system. In this way, when the test operation is carried out in the safety cabinet, the aerosol or dangerous particles released by the sample do not spread to the surroundings or escape to the window due to the downward airflow in the cabinet and the intake air flow in the front window and the negative pressure at the bottom of the operating area in the cabinet. It is directly sucked into the negative pressure passage at the bottom of the safety cabinet, cleaned by the high-efficiency filter, or partially recycled back to the cabinet or discharged to the environment. Due to the double-layer structure of the cabinet, even if the inner wall of the operation area leaks, the contaminated gas will be trapped by the negative pressure ventilation system of the surrounding negative pressure passage and will not be directly discharged into the environment. At the same time, the standard also stipulates that the three side wall panels in the bare working area of ​​the safety cabinet should be in one-piece structure, and the joints of the inner surface must be sealed. The working area of ​​the Heal Force safety cabinet adopts a three-sided stainless steel integrated double-layer structure, and adopts a 8 mm large rounded transition treatment at the junction. The advantage of this is that all the contaminated parts in the safety cabinet working area have no hidden danger factors. It is possible that all of the aerosol particles produced by the test will be filtered by the negative pressure airflow into the negative pressure ventilation system, and it is also very convenient to clean and disinfect the cabinet.

3. There are two methods for the anti-leakage performance test of the cabinet of the biosafety cabinet. One is the pressure decay test, which closes the front window and the exhaust vent of the safety cabinet, and pressurizes the cabinet to 500Pa for 30 minutes. Connect the pressure gauge or pressure sensor system to the test area to display the internal pressure, and the air pressure in the cabinet is required to be no less than 450Pa. The pressure decay method is used to test the anti-leakage performance of the biosafety cabinet. It is a test method with accurate and high requirements. The airtightness of the biosafety cabinet and the overall design of the cabinet are very high. It is also included in China YY0569-2005. The test method recommended by the US NSF49 and other biosafety cabinet standards; the other is the soap bubble test, that is, spraying or painting along the outer surface of all welds, sockets and seals of the safety cabinet pressure ventilation system. Leak the soap solution and pressurize the cabinet to 500pa to see if there is any air bubbles. If a big leak occurs, it can be found by a slight airflow feeling and sound. Although the soap bubble method is also recognized by the Chinese YY0569-2005 standard, its test effect is far less accurate and reliable than the pressure decay method. It is hoped that when the teacher chooses the safety cabinet, it must be clear how the manufacturer conducts the cabinet leak prevention test. Heal Force safety cabinet is the only safety cabinet brand in China that has passed the strict cabinet anti-leakage pressure decay method. The excellent air tightness ensures the possibility of no leakage of polluting gas and particles, so that safety can be avoided. . 4. Optimized airflow rate and excellent fan China YY0569-2005 standard specifies that the low average flow velocity of the inflow of the front window operating port of the safety cabinet should be 0.50 m/s, and the average flow rate for the descending airflow should be 0.25 m/s and 0.5. Between m/s, and to dynamically display the inflow and descending airflow rates of the safety cabinet in real time on the control panel. Such a flow velocity ensures that the airflow blocks the dangerous particles such as aerosols generated during the test from spreading or escaping. The aerosol and particles are sucked into the negative pressure exhaust system by the combined inflow and descending airflows. Filtered out. The front window of the Heal Force safety cabinet has an inflow velocity of 0.53 m/s and a descending airflow velocity of 0.35 m/s, which ensures that the airflow velocity in the cabinet reaches the ratio of the good ratio of the Zui, and the two airflows can be dynamically displayed on the control panel in real time. The flow rate allows the operator to know the wind speed of the safety cabinet at all times. Once the inflow or descending air flow rate is lower than or higher than the nominal value of the flow rate specified by the manufacturer, the safety cabinet will sound and light alarm, which will better protect personnel and protect the product. The role.

5. Excellent self-inductive fan In the safety of the safety cabinet, in addition to high efficiency filter and perfect cabinet structure and good cabinet air tightness, another important guarantee is the reliable performance of the fan. As the use time of the safety cabinet increases, the amount of particles accumulated on the surface of the filter membrane also increases, and a large resistance to the passing air is generated, so that the flow rate and flow rate of the airflow in the cabinet are weakened, and the safety cabinet fan is specified in the standard. When the safety cabinet is in normal operation, without adjusting the motor power in the fan, when the wind pressure of the filter drops by 50%, the exhaust air volume of the fan should not fall by more than 10%. It is also stipulated that the system should be equipped with a fan speed control device. Respond to the loss of exhaust air volume. The Heal Force safety cabinet adopts the world-famous self-inducting fan that can automatically adjust the motor power in the configuration of the fan. It can automatically increase the power of the fan according to the pressure of the high-efficiency filter to increase the air volume and ensure the inside of the cabinet. The airflow rate and wind pressure are always in a safe range, avoiding the threat to the safety performance of the safety cabinet when the airflow rate and air volume in the cabinet are lost.
6. The necessary alarm prompt function When purchasing the safety cabinet, in addition to the above-mentioned hard indicators of the safety performance of the safety cabinet, there is also a soft indicator, which is the necessary alarm function of the safety cabinet. . Through the above introduction, we know that in order to ensure the real "safety" of the biosafety cabinet, we have made necessary and reasonable optimization in the material selection, structural design and various key components of the safety cabinet, but in actual use, the safety cabinet There may be "unsafe" hazards due to various reasons, such as machine failure, human error or other reasons. When this happens, the operator may not be aware of it. In response to this situation, China's YY0569-2005 industry standard stipulates that the safety cabinet must have various alarm functions that may cause hidden dangers. For example, if the opening height of the front window of the safety cabinet exceeds the specified height, the alarm will be issued. When the downward airflow or the inflow airflow in the cabinet does not reach the specified flow rate, the alarm will be issued. The safety cabinet with the dual fan must have the interlocking of the falling fan and the exhaust fan. Alarms, etc.

Above, we have expounded several key points that should be noted when purchasing a biosafety cabinet to ensure the safety of the safety cabinet. However, if only the technical indicators specified in the industry standards are met and there is no deep understanding of these parameters, it is difficult to make various corresponding optimization designs for the safety cabinets for various problems encountered in practical applications. The safety cabinet is completely guaranteed to be truly safe. Therefore, we recommend that when purchasing a biosafety cabinet, you should not only see if the safety cabinet has reached the industry standard, but also whether the manufacturer of the production safety cabinet has accumulated long-term technical experience in this field, and thus it is safe. The cabinet performs key technical optimizations. Because this is the only way to ensure that the biosafety cabinet is truly safe.

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